ISODEOXYELEPHANTOPIN (IDOE) RETARDS TUMOR CELL MIGRATION BY DOWNREGULATING MMP-2/9 EXPRESSION IN TRIPLE NEGATIVE BREAST CANCER

Abstract

Introduction: Isodeoxyelephantopin (C19H20O6), plant-derived sesquiterpene, extracted from Elephantopus Scaber Linn., have tremendous contributions in inhibiting the proliferation and metastasis of cancer cells.

Materials and Methods: The cell viability assay was shown to be procured half-maximal inhibitory concentration of isodeoxyelephantopin on the MDA-MB-231cell line. Apoptosis was screened by AO/ EB staining and it was confirmed by annexin V staining. The DNA damaging property of IDOE was examined by comet assay. The cell cycle arrest determined using flow cytometry analysis and the growth inhibition due to the modulation of cell cycle regulatory protein p53 were substantiated by RT PCR. Scratch wound migratory assay was done to evaluate the migratory effect of IDOE on the MDA-MB-231 cell line. RT-PCR analysis showed that expression of MMP-2/9 was inhibited by IDOE treatment.

Result: Anti-proliferative and anti-migratory effect of IDOE was determined by cell cycle analysis and annexin v staining. Cells were arrested in the G2/M phase and it is due to the modulation of cell cycle regulatory protein p53. The mRNA level expression studies of matrix metalloproteinases, MMP-2/9 provide supporting data to prove the anti-migratory property.